BD FACSAria II 桌上型高速流式細胞分選儀
毋需光路調整！
l 採用固態雷射固定式光束成形系統，搭配全新設計之Flow Cell逹到精準照射位置。因此，不需要繁複的雷射光路調整作業。
自動液滴調控機能！
l 新開發SweetSpot系統（專利申請中），能監測最佳化液滴形成狀態，並自動調控液滴振盪頻率，重現並維持液滴形成最佳状態，逹到精確的細胞分選功能。
自動樣品保護功能！
l SweetSpot系統能感知液流系統中阻塞物的形成，並能自動關閉分選機制，保護已分選下來的珍貴樣品，避免因阻塞所造成的汚染。
四向高速分選為標準裝備QudraSortTM！
l 一次可同時分選四群或二群不同特性的細胞群。可應用雙向、四向分選器、或鏡檢用玻片承收。可選配Auto Cell Deposit Unit系統使分選出的細胞可用96孔或384孔盤進行選殖性（Cloning）細胞分選，每個孔可分選1~10萬個細胞。
Octagon八向分光系統，提昇細胞解析能力！
l 新開發之Octagon八向、Trigon三向分光系統（專利申請中），運用「反射效能優於透射」的光學原理，有效地將螢光信號八向波形迴路處理，使單支雷射最多可解析七色螢光、及一個散射光信號，配合三支雷射則最多可同時解析十三色螢光信號、及兩個散射光信號。
新開發Flow Cell，實現高速分選美夢！
l 新開發之高感度Rectangle石英杯組合式噴嘴系統（專利申請中），1秒間可振盪形成90,000液滴，實現研究人員高速分選的夢想。每秒鐘儀器最高可分析70,000個細胞，並從中分選出30,000個有興趣細胞。
New 375-nm Near UV laser
The 375-nm Near UV laser option is available to expand the range of application choices. The beam geometry is optimized for most hematopoietic stem cells. The top hat design of the 375-nm Near UV laser triples the amount of light at the sample intercept. The 375-nm Near UV laser can be operated together with the red and blue lasers.
New fluidics and nozzle designs
A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted and new nozzle design allows for reproducible insertions. Software wizards and automation simplify aseptic sort setup, drop delay determination, and clog detection.
Better workflow and productivity
The BD FACSAria II also raises the bar on ease of use to improve both workflow and productivity with improvements across the platform. For example, a universal sort tube collection mount now makes it easier to insert and remove tube holders. In addition, software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.
BD FACSAria Cell Sorting System
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First benchtop high-speed sorter with fixed-alignment cuvette flow cell |
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Cuvette flow cell for superior fluorescence sensitivity |
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Up to three air-cooled lasers at 488-nm, 633-nm, and 407-nm wavelengths |
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Digital acquisition rates of up to 70,000 events/second |
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Multicolor analysis of up to 15 parameters |
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Two- and four-way bulk sorting devices for a variety of tube sizes |
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Optional BDTM Automated Cell Deposition Unit (ACDU) for sorting to BD MultiwellTM plates or microscope slides |
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Available BDTM Aerosol Management Option |
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No special facility requirements necessary |
BD LSR™
II flow cytometer
Building on the ease of use and flexibility of the BD™ LSR, the first UV
benchtop analyzer, BD Biosciences introduces the BD™ LSR II. The BD LSR II has
performance, capabilities, and flexibility never seen before in a benchtop
analyzer. Free up your high-performance cell sorter by moving your
application(s) that require a UV laser to the BD LSR II. The BD LSR II can be configured with up to four fixed alignment lasers and
the ability to detect up to 18 colors, utilizing a revolutionary optical design.
The BD LSR II can be configured with a 488 nm, 405 nm, 633 nm, and 355nm UV
laser. At the heart of the BD LSR II are new digital electronics. The
electronics digitize signals 10 million times per second in 16,384 discrete
levels. This expands channel resolution and increases detection sensitivity.
Electronic dead time is eliminated; more precise fluorescent measurements can be
made improving linearity, compensation, and quantitation of fluorescence
measurements.Four-laser, 18-color benchtop flow cytometer
BD LSR II System
Laser Options
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BD FACSCanto™ II flow cytometer |
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BD has been innovating flow cytometry since the release of the first commercial flow cytometer in 1974. That tradition continues with the new BD FACSCanto™ benchtop flow cytometer. Its key features are:
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FACS Calibur flow cytometry
一、簡介
是全自動四色桌上型分選儀，它集分析分選功能於一身，配置靈活，結合自動化的樣本處理系統，試劑選擇範圍廣，強力電腦軟體分析系統，絕對配合現代實驗室高產能的需要。性能卓越，不僅通過友好的使用介面，幫助醫檢師快速從事常規淋巴球亞群免疫表型、DNA、血小板、絕對計數等臨床分析，更以其完美的四色分析和獨特的分選功能，成為免疫功能、血液、腫瘤、遺傳等諸多研究領域不可或缺的重要工具。
˙開機無需等待
暖機5分鐘即可上機檢測。
˙自動化程度高
FACSCalibur 以用戶為中心的系統設計，從自動化樣本處理、自動上樣，到按鈕式液流控制、自動化軟體獲取和分析，每個環節操作都簡便快捷，並提供準確客觀且具高重現性實驗結果。
˙分析速度快
FACSCalibur 螢光檢測全部採用大型機優勢配置--光電倍增管(PMT)以保證最快的分析速度每秒 10000 個細胞。FACSCalibur 是目前世界上分析速度最快的桌上型流式細胞儀。
˙完美的四色分析系統
BD專利的立體空間激發系統通過雙雷射實現 4 色分析，大大地減少不同雷射間產生螢光信號之光補償調節，提高檢測靈敏度；同時雙雷射的應用大大擴展了螢光染劑的選擇範圍，相對地擴大了儀器的應用領域。雙雷射立體空間激發系統為流式細胞儀四色分析設立了行業標準。
˙完美的四色分析系統
FACSCalibur 是目前世界上唯一的桌上型分選儀。
˙完美的四色分析系統
FACSCalibur 用戶至上的設計理念的又一體現。用戶可以根據實際需要和預算情況選配第二根雷射、分選及濃集系統、自動進樣系統、96 孔盤自動進樣系統。
˙DNA 分析的解決之道
DDM(Doublet Discrimination Module) 雙連體辨別模式，透過 FL2-W 和 FL2-A 的檢測，可區別實體組織標本中的雙連細胞群體，大大降低假陽性發生機率，真實呈現單一細胞 DNA 套體分析結果。
˙唯一的雙參數閾值設定
可將雜訊減至最低，增加獲取分析速度，使微量細胞檢測成為可能。
Application of FACSCalibur Sorting
Applications...
Cell Subsetting
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Tissue Preparation for Surface Antigen Staining
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Four-Color Analysis of Bone Marrow Transplantation Specimens
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Progenitor Cell Subsetting with ProCOUNT
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Running Simultest Samples Using the FACS Loader
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Cell Proliferation and Cytokine Detection
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Detecting Cytokines in Antigen-Activated Lymphocytes
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Detecting Cytokines in Lymphocytes
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Detecting Cytokines in Monocytes
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» New Flurochromes and Instrumentation for Multicolor Flow Cytometry
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Dendritic Cell CD123+ and CD11c+ Subsets
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Isolation of DNA |
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In Situ Hybridization and Image Analysis on Nuclei Sorted on the Basis
of DNA Content |
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Selected References: Flow Cytometry as a Tool for Proteomics
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