BD™ CARV II                                                                       

BD™ CARV II Confocal Imager 即時活細胞多色共軛焦影像分析儀

　

CARV II共軛焦影像分析儀，乃是將簡易操作和經濟有效的特殊光學套件，整合裝配在現有的顯微鏡上，以產生高解析度的共軛焦影像，並能夠進行擷取與儲存。

高速度多點的共軛焦掃描, 與高量子分辨率CCD照相機結合, 使 photobleaching 減到最少，而且，即時的影像擷取和記錄，可達到1000個掃瞄/秒。長效弧燈光源透過一個事先校準的光導，整合到儀器中，將可以進行全光譜(360-700nm)任何真實螢光的共軛焦影像擷取。內建多種不同的激發,雙色分光和放射之光學濾鏡轉盤，將會對相同的樣品進行自動化的快速多方向影像擷取，最高可達到五種或者更多種的螢光共軛焦影像擷取。

　

CARV II共焦點影像分析儀，其系統能夠應用於許多專業特殊的CCD照相機與許多商業的影像分析軟體，也能夠提供 fluorescence recovery after photobleaching (FRAP)研究的自動化與分析。

　

BD™ CARV II offers:

Multipoint Confocal Scanning
The BD™ CARV II Confocal Imager module utilizes a Nipkow spinning disk which contains multiple sets of spirally arranged pinholes placed in the image plane of the objective lens. The column of excitation light is split into 1000 beams to simultaneously scan the entire field at a rate of 1000 times per second, effectively creating a full image of the focal plane in real-time. Emitted light is collected and imaged using a high resolution and high quantum efficiency CCD camera.

The significant advantages to the spinning disk approach are the ability to monitor rapidly occurring events within living cells without compromising resolution, as well as the high frequency low intensity illumination substantially reduces photobleaching and phototoxicity.

Confocal Microscopy

Direct Viewing and Imaging of Confocal and Wide Field
The BD™ CARV II Confocal Imager permits direct viewing of confocal images through a binocular eyepiece and/or through the camera for fast imaging setup. It is the only pinhole spinning disk fluorescence confocal systems which allows the user to quickly switch from confocal to wide-field viewing or recording.

Wide FieldConfocal
　

                                                    Sea Urchin Egg (Tubulin), Friday Harbor, UW George von Dassow, Ph.D.

Full Spectrum Confocal
The BD™ CARV II uses a Halide/Mercury Halide Arc lamp as an illumination source. This allows full spectrum (360nm-700nm), real-time confocal imaging. In combination with the vast array of commercially available filter sets, any fluorescent marker can be confocally imaged at a fraction of the cost of laser based systems.

Automated filter selection
Automation of internal multi-position excitation (8), dichroic (5) and emission (8) filter wheels allows fast multi-dimensional confocal imaging. The automated filters reduce the reliance on multi-band pass filter sets allowing maximum light throughput and fast sequential imaging of up to five or more fluorescent probes in the same sample.

Fluorescence Recovery After Photobleaching (FRAP) capabilities
A FRAP Iris which is at the same focal plane as the confocal disk and creates an adjustable rectangular aperture on the image. This enables controlled photo-bleaching of part of the sample with high intensity Hg/metal halide light followed by fluorescence recovery recording.

Microscope Compatibility

The BD™ CARV II unit can be configured to most inverted and upright fluorescent microscopes.

Application Specific Cameras
A wide selection of high-end cooled and non-cooled CCD cameras with a combination of 12- 16 bit information, fast readout, high quantum efficiencies and small pixel sizes produces images at a high resolution and high signal-to-noise ratio.

3D Software Option

A range of state of the art 3D software packages can be used for acquisition and analysis of confocal images.

3D Reconstruction

3D Reconstruction

elegcarv2 Cell division
60X 1.4 NA Olympus
Sensicam QE (Cooke)
GFP Tubulin
3D reconstruction
IP Lab		 embryo_4 GFP- Neurons
60X 1.4 NA Olympus
Sensicam QE
IP Lab

　

PartialA3drgb1 Tubulin
60X 1.4 NA Nikon
CCD-CoolSNAP HQ
z stack 0.4 micron steps
Dr George von Dassow
Friday Harbor, UW
MetaMorph		 desmid3d Auto-fluorescence
60X 1.4 NA Olympus
ORCA ER
MetaMorph


　

seaurchin_3d Sea Urchin
Tubulin
60X 1.4 NA Nikon
CCD-CoolSNAP HQ
z stack 0.4 micron steps
Dr George von Dassow
Friday Harbor, UW
MetaMorph		 seaurchin_3dstack Sea Urchin
Tubulin
60X 1.4 NA Nikon
CCD-CoolSNAP HQ
z stack 0.4 micron steps
Dr George von Dassow
Friday Harbor, UW
MetaMorph


　

Skin Skin
DAPI, FITC, Tex Red,
60X 1.4 NA Olympus
ORCA 100
Kenedy & Crabb,
Uni. of Minnesota		 Inner ear hair cell Inner ear hair cell
FITC
100X 1.4 NA Olympus
CCD- ORCA ER
0.4 micron steps (25)
Dr Matthew Kelley
NIDCD/NIH


　

skin3D FITC, Tex Red, Cy5
60X 1.4 NA Olympus
ORCA 100
Kenedy & Crabb,
University of Minnesota

　

Time Laspe
Using any recommended imaging software and camera, time lapse imaging of cells or organisms can be performed for long periods of time without significant bleaching or phototoxicity. Depending on the speed of the physiology being measured, with the right camera and z-stepper combination, time lapse at a single plain or at multiple plains (4D) can be imaged.

　

Microtubule Dynamics

　

Long Term Time Lapse

f_13z_frames_dicty4 GFP - 4D
100X 1.4NA Olympus
150ms, 13 Z steps, 30min
CCD- Cascade 512B
Dr. David Knecht Department of Molecular and Cell Biology
University of Connecticut.		 25gfp GFP-Caveolin
60X 1.4 NA Olympus
ORCA ER


　

32gfp GFP-Caveolin
60X 1.4 NA Olympus
ORCA ER		 er2 60 h20 worm Nematode worm(C.elegans)
Cell division
60X 1.3 water
CCD-CoolSNAP HQ
Single plain time lapse
300ms every 4 s
Friday Harbor, UW
MetaMorph

　

drosophila_tubulin2 GFP- Tubulin
60X 1.4NA Olympus
CCD- ORCA ER
Dr Nico Stuurman
UCSF		 drosophila_tubulin3 GFP- Tubulin
60X 1.4NA Olympus
CCD- ORCA ER
Dr Nico Stuurman
UCSF

　

tubulin_histone1 Nematode C. elegans
Cell division
60X 1.4 NA Nikon
CCD-CoolSNAP HQ
Single plane time lapse
15 min, 300ms every 4 s
Friday Harbor, UW
MetaMorph

　

　

High Speed Calcium Imaging
BD™ CARV II, in combination with intensified cameras and cameras with on chip amplification, can be used to image fast fluorescence changes at rates ranging from 50-100 frames per second.

　　　

High Speed Calcium Imaging

　

BD™ CARV II can be configured to a variety of inverted microscopes.

Nikon Olympus Leica Zeiss

TE 2000

TE 200/300

TE 2000 series

IX 1 Series

IX 2 Series

 

 DMR series Axiovert 200

　

Publication List for BD CARV II Confocal Imager

　

Robert P. Brendza, Brian J. Bacskai, John R. Cirrito, Kelly A. Simmons, Jesse M. Skoch, William E. Klunk, Chester A. Mathis, Kelly R. Bales, Steven M. Paul, Bradley T. Hyman, and David M. Holtzman
Anti-Aß antibody treatment promotes the rapid recovery of amyloid-associated neuritic dystrophy in PDAPP transgenic mice
J. Clin. Invest., Feb 2005; 115: 428 - 433.

Sophie Layalle, Elise Coessens, Alain Ghysen, and Christine Dambly-Chaudière
Smooth, a hnRNP encoding gene, controls axonal navigation in Drosophila
Genes Cells, Feb 2005; 10: 119 - 125.

Juan Domínguez-Bendala, Dagmar Klein, Melina Ribeiro, Camillo Ricordi, Luca Inverardi, Ricardo Pastori, and Helena Edlund
TAT-Mediated Neurogenin 3 Protein Transduction Stimulates Pancreatic Endocrine Differentiation In Vitro
Diabetes, Mar 2005; 54: 720 - 726.

Simon Collier, Haeryun Lee, Rosemary Burgess, and Paul Adler
The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis
Genetics, Apr 2005; 169: 2035 - 2045.

Shannon H. Cole, Ginger E. Carney, Colleen A. McClung, Stacey S. Willard, Barbara J. Taylor, and Jay Hirsh
Two Functional but Noncomplementing Drosophila Tyrosine Decarboxylase Genes: DISTINCT ROLES FOR NEURAL TYRAMINE AND OCTOPAMINE IN FEMALE FERTILITY
J. Biol. Chem., Apr 2005; 280: 14948 - 14955.

Ayana T. Moore, Kathleen E. Rankin, George von Dassow, Leticia Peris, Michael Wagenbach, Yulia Ovechkina, Annie Andrieux, Didier Job, and Linda Wordeman
MCAK associates with the tips of polymerizing microtubules
J. Cell Biol., May 2005; 169: 391 - 397.

Nan Ren, Chunming Zhu, Haeryun Lee, and Paul N Adler
Gene Expression During Drosophila Wing Morphogenesis and Differentiation
Genetics, Jul 2005; doi:10.1534/genetics.105.043687

William M. Bement, Hélène A. Benink, and George von Dassow
A microtubule-dependent zone of active RhoA during cleavage plane specification
J. Cell Biol., Jul 2005; 170: 91 - 101.

Luis R. Landa, Jr., Mark Harbeck, Kelly Kaihara, Oleg Chepurny, Kajorn Kitiphongspattana, Oliver Graf, Viacheslav O. Nikolaev, Martin J. Lohse, George G. Holz, and Michael W. Roe
Interplay of Ca2+ and cAMP Signaling in the Insulin-secreting MIN6 -Cell Line
J. Biol. Chem., Sep 2005; 280: 31294 - 31302.

P. Iserovich, J. Li, A. Cheng, L. Ma, K. Kuang, and J. Fischbarg
Route of Fluid Transport Across Corneal Endothelium From the Observation of Movement of Microscopic Particles
Invest. Ophthalmol. Vis. Sci., May 2005; 46: 2205.


Juan Domínguez-Bendala, Dagmar Klein, Melina Ribeiro, Camillo Ricordi, Luca Inverardi, Ricardo Pastori, and Helena Edlund
TAT-Mediated Neurogenin 3 Protein Transduction Stimulates Pancreatic Endocrine Differentiation In Vitro
Diabetes, Mar 2005; 54: 720 - 726.


Simon Collier, Haeryun Lee, Rosemary Burgess, and Paul Adler
The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis
Genetics, Apr 2005; 169: 2035 - 2045.


P. Iserovich, J. Li, A. Cheng, L. Ma, K. Kuang, and J. Fischbarg
Route of Fluid Transport Across Corneal Endothelium From the Observation of Movement of Microscopic Particles
Invest. Ophthalmol. Vis. Sci., May 2005; 46: 2205.

P. Iserovich, J. Li, A. Cheng, L. Ma, K. Kuang, and J. Fischbarg
Route of Fluid Transport Across Corneal Endothelium From the Observation of Movement of Microscopic Particles
Invest. Ophthalmol. Vis. Sci., May 2005; 46: 2205.

Ayana T. Moore, Kathleen E. Rankin, George von Dassow, Leticia Peris, Michael Wagenbach, Yulia Ovechkina, Annie Andrieux, Didier Job, and Linda Wordeman
MCAK associates with the tips of polymerizing microtubules
J. Cell Biol., May 2005; 169: 391 - 397.

Michal Kozubek, Petr Matula, Pavel Matula, Stanislav Kozubek
Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy (p 164-175) Published Online: 18 Aug 2004 Microscopy Research and Technique Volume 64, Issue 2 Special Issue: Image Processing in Optical Microscopy . Issue Edited by Alberto Diaspro., Originally published as MBC in Press, 10.1091/mbc.E04-09-0828 on December 9, 2004 . Vol. 16, Issue 2, 689-700, February 2005

Ying He *, Xiaolan Fang *, Kazuo Emoto , Yuh-Nung Jan , and Paul N. Adler * 

The Tricornered Ser/Thr Protein Kinase Is Regulated by Phosphorylation and Interacts with Furry during Drosophila Wing Hair Development

 

 

Shannon H. Cole, Ginger E. Carney, Colleen A. McClung, Stacey S. Willard, Barbara J. Taylor, and Jay Hirsh
Two Functional but Noncomplementing Drosophila Tyrosine Decarboxylase Genes: DISTINCT ROLES FOR NEURAL TYRAMINE AND OCTOPAMINE IN FEMALE FERTILITY
J. Biol. Chem., Apr 2005; 280: 14948 - 14955.

 

Ayana T. Moore1, Kathleen E. Rankin1, George von Dassow2, Leticia Peris3, Michael Wagenbach1, Yulia Ovechkina1, Annie Andrieux3, Didier Job3, and Linda Wordeman1

MCAK associates with the tips of polymerizing microtubules

Published 9 May 2005. doi:10.1083/jcb.200411089

© The Rockefeller University Press, 0021-9525

JCB, Volume 169, Number 3, 391-397

 

 

Agnès Bonnot, George Z. Mentis, Jesse Skoch, and Michael J. O'Donovan
Electroporation Loading of Calcium-Sensitive Dyes Into the CNS
J Neurophysiol, Mar 2005; 93: 1793 – 1808

 

Stephen J. Perry, Sachiko Junger, Trudy A. Kohout, Sam R. J. Hoare, R. Scott Struthers, Dimitri E. Grigoriadis, and Richard A. Maki
 Distinct Conformations of the Corticotropin Releasing Factor Type 1 Receptor Adopted following Agonist and Antagonist Binding Are Differentially Regulated
J. Biol. Chem., Mar 2005; 280: 11560 - 11568.

Sharon C. Braunagel, Shawn T. Williamson, Suraj Saksena, Zhenping Zhong, William K. Russell, David H. Russell, and Max D. Summers
Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins: Facilitated trafficking to the inner nuclear membrane
PNAS, Jun 2004; 101: 8372 - 8377.

Laszlo Karai, Dorothy C. Brown, Andrew J. Mannes, Stephen T. Connelly, Jacob Brown, Michael Gandal, Ofer M. Wellisch, John K. Neubert, Zoltan Olah, and Michael J. Iadarola
Deletion of vanilloid receptor 1_expressing primary afferent neurons for pain control
J. Clin. Invest., May 2004; 113: 1344 - 1352.

Max J. Hilz, Felicia B. Axelrod, Andreas Bickel, Brigitte Stemper, Miroslaw Brys, Gwen Wendelschafer-Crabb, and William R. Kennedy
Assessing function and pathology in familial dysautonomia: assessment of temperature perception, sweating and cutaneous innervation
Brain, Sep 2004; 127: 2090 - 2098.

Deborah A. Scheuer, Andrea G. Bechtold, Sylvan S. Shank, and Susan F. Akana
Glucocorticoids act in the dorsal hindbrain to increase arterial pressure
Am J Physiol Heart Circ Physiol, Jan 2004; 286: 458 - 467.

Linda C. Baker, Robert Wolk, Bum-Rak Choi, Simon Watkins, Patricia Plan, Anisha Shah, and Guy Salama
Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse hearts
Am J Physiol Heart Circ Physiol, Oct 2004; 287: H1771 - H1779

 

Gail M. Verge1, Erin D. Milligan2, Steve F. Maier2, Linda R. Watkins2, Gregory S. Naeve1, * and Alan C. Foster1

Fractalkine (CX3CL1) and fractalkine receptor (CX3CR1) distribution in spinal cord and dorsal root ganglia under basal and neuropathic pain conditions

European Journal of Neuroscience

Volume 20 Issue 5 Page 1150  - September 2004

doi:10.1111/j.1460-9568.2004.03593.x

 

Josefa Andrade, Hu Zhao, Brian Titus, Sandra Timm Pearce, and Margarida Barroso
The EF-Hand Ca2+-binding Protein p22 Plays a Role in Microtubule and Endoplasmic Reticulum Organization and Dynamics with Distinct Ca2+-binding Requirements
Mol. Biol. Cell, Feb 2004; 15: 481 - 496.

 

Manami Hara, Vytautas Bindokas, James P. Lopez, Kelly Kaihara, Luis R. Landa, Jr., Mark Harbeck, and Michael W. Roe
Imaging endoplasmic reticulum calcium with a fluorescent biosensor in transgenic mice
Am J Physiol Cell Physiol, Oct 2004; 287: C932 - C938.

Peter D. Yurchenco, Yi-Shan Cheng, Kevin Campbell, and Shaohua Li
Loss of basement membrane, receptor and cytoskeletal lattices in a laminin-deficient muscular dystrophy
J. Cell Sci., Feb 2004; 117: 735 - 742.

BEHAVIORAL/SYSTEMS/COGNITIVE:
 I. A. Khasabova, C. Harding-Rose, D. A. Simone, and V. S. Seybold
Differential Effects of CB1 and Opioid Agonists on Two Populations of Adult Rat Dorsal Root Ganglion Neurons
J. Neurosci., Feb 2004; 24: 1744 - 1753.

Linda C. Baker, Robert Wolk, Bum-Rak Choi, Simon Watkins, Patricia Plan, Anisha Shah, and Guy Salama
Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse hearts
Am J Physiol Heart Circ Physiol, Oct 2004; 287: H1771 - H1779.

 

Drosophila Bys is nuclear and shows dynamic tissue-specific expression during development

Mary J. Stewart1  and Erik K. Nordquist

Development Genes and Evolution

Publisher: Springer-Verlag GmbH

ISSN: 0949-944X (Paper) 1432-041X (Online)

DOI: 10.1007/s00427-004-0447-8

Issue:  Volume 215, Number 2

Date:  February 2005

Pages: 97 - 102

Provitera V.1; Nolano M.1; Stancanelli A.1; Lullo F.1; Santoro L.2

Standard deviation of the distances between consecutive epidermal nerve fibers as a parameter to study their distribution in human skin

Journal of the Peripheral Nervous System, June 2004, vol. 9, no. 2, pp. 114-114(1)

 

Sharon C. Braunagel, Shawn T. Williamson, Suraj Saksena, Zhenping Zhong, William K. Russell, David H. Russell, and Max D. Summers
Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins: Facilitated trafficking to the inner nuclear membrane
PNAS, Jun 2004; 101: 8372 - 8377.

 

Emanuela Galliera, Venkatakrishna R. Jala, John O. Trent, Raffaella Bonecchi, Paola Signorelli, Robert J. Lefkowitz, Alberto Mantovani, Massimo Locati, and Bodduluri Haribabu
-Arrestin-dependent Constitutive Internalization of the Human Chemokine Decoy Receptor D6
J. Biol. Chem., Jun 2004; 279: 25590 - 25597.

 

Carine Bonnon, Laurence Goutebroze, Natasha Denisenko-Nehrbass, Jean-Antoine Girault, and Catherine Faivre-Sarrailh
The Paranodal Complex of F3/Contactin and Caspr/Paranodin Traffics to the Cell Surface via a Non-conventional Pathway
J. Biol. Chem., Nov 2003; 278: 48339 - 48347.

 

Sean P. Marrelli, Maxim S. Eckmann, and Michael S. Hunte
Role of endothelial intermediate conductance KCa channels in cerebral EDHF-mediated dilations
Am J Physiol Heart Circ Physiol, Oct 2003; 285: 1590 – 1599

Sean P. Marrelli, Maxim S. Eckmann, and Michael S. Hunte
Role of endothelial intermediate conductance KCa channels in cerebral EDHF-mediated dilations
Am J Physiol Heart Circ Physiol, Oct 2003; 285: 1590 - 1599.

Carine Bonnon, Laurence Goutebroze, Natasha Denisenko-Nehrbass, Jean-Antoine Girault, and Catherine Faivre-Sarrailh
The Paranodal Complex of F3/Contactin and Caspr/Paranodin Traffics to the Cell Surface via a Non-conventional Pathway
J. Biol. Chem., Nov 2003; 278: 48339 - 48347.

Rachell E. Booth, Qiusheng Tong, Jorge Medina, Peter M. Snyder, Pravina Patel, and James D. Stockand
A Region Directly Following the Second Transmembrane Domain in ENaC Is Required for Normal Channel Gating
J. Biol. Chem., Oct 2003; 278: 41367 - 41379.