High Content Bioimagers

高內涵活細胞共焦影像分析儀

                                                                                                          

 

結合流式細胞分析的完整細胞研究解決方案

流式細胞技術是一種利用螢光來標定細胞,並進行快速自動分析及分選被螢光標定細胞的專門技術,可是無法確知相關於細胞大小、形態及移動狀況。而高內涵螢光影像技術,研究者無需將細胞從培養的環境移取進行製備,便可了解細胞反應移動的真實情形,若結合此二種先進技術,將可提供細胞研究者最完整且快速的解決方 
 

優點:

流式細胞技術 高內涵螢光影像技術
項目符號 透過螢光強度的表現進行細胞群體的統計分析
項目符號 應用光電倍數管偵測技術提供單細胞層次的解析
項目符號 進行數以萬計細胞的快速分析
項目符號 細胞必需製備在懸浮液中
項目符號 多螢光參數分析(3~18 colors)
項目符號 可進行細胞分選(sorting)

 

項目符號 螢光強度、細胞大小、形態、移動狀況及Localization
項目符號 應用高解析CCD偵測技術提供次細胞層次的解析(Subcellular resolution)
項目符號 進行數以千計細胞的快速分析
項目符號 最適用於貼附細胞及組織細胞
項目符號 多螢光參數分析(3~8 colors)
項目符號 可長時重覆觀察

 

 

BD Pathway™ 400 Series

BD Biosciences – Bioimaging Systems introduces the Pathway 400 series of automated imaging instruments for high-content cellular analysis.

BD Pathway™ 435 confocal bioimager

BD Pathway™ 435 confocal bioimager

The BD Pathway™ 435 is an affordable, compact benchtop imager compatible with multiwell plates and slides, providing a broad array of automated imaging capabilities. The system rapidly acquires high-resolution images with high-performance laser autofocus, true confocal optics, flexible computer-controlled excitation and emission filter changers, and a long-life alignment-free metal halide lamp.

With updated image analysis software featuring new tools and wizard-style applications, the BD Pathway™ 435 confocal bioimager simplifies the process of high-content cellular analysis.

The system enables a broad array of fluorescence-based biological applications* such as neurite outgrowth analysis, angiogenesis, protein translocation, colocalization, cell cycle, and 3-dimensional cellular exploration. The BD Pathway™ 435 is ideal for researchers that need high-speed, high-resolution confocal imaging capabilities coupled with powerful and flexible image analysis.

BD Pathway™ 415 bioimager

For applications not requiring confocal, the BD Pathway™ 415 is a cost effective alternative to the Pathway 435. And, the Pathway 415 can be field-upgraded to confocal if later applications require it.

 

Highlights of the Pathway 415 and 435 Bioimagers

 

項目符號 High-performance, laser-based autofocus is standard. You can elect to use the laser autofocus, camera autofocus, or a combination of both.
項目符號 Robot-compatible, retractable plate tray permits imaging even in well-lit laboratories.
項目符號 Transmitted light canopy provides illumination for brightfield imaging.
項目符號 User accessible filters allow easy filter wheel exchange for a broader range of imaging options and customization.
項目符號 The Pathway 435 adds BD’s proprietary spinning disk confocal capability and associated 3D imaging software.
項目符號 High quantum efficiency cooled 12 bit CCD camera produces high resolution images for analysis.
項目符號 Flexible illumination using 8 excitation, 8 emission, and 5 dichroic filters that are all independently controlled.
項目符號 Compact unit with integrated electronics and vibration dampening system sits on top of a laboratory bench.
項目符號 Accurate x, y, z positioning provides an optimal environment for precision imaging.

 

 

 

 

 

 

 

 

BD Pathway 855 Bioimager

Explore cells in real-time and develop assays faster with the power and flexibility of the BD Pathway™ Bioimager

Pathway HT (click photo for large image)

The BD Pathway Bioimager is an automated, confocal, real-time, single cell kinetic and endpoint imaging system that has been integrated into a single, compact unit. The BD Pathway was designed to provide high-resolution, automated confocal imaging with sophisticated imaging software to assist in developing better cell-based assays. The system allows the user to explore biological events without many of the restrictions of conventional microscope-based high content imaging systems.

Learn more about the BD Pathway Bioimager »

 

 

Highlights of the BD Pathway 855 Bioimager

 

項目符號 Ability to run kinetic and endpoint assay formats
項目符號 Integrated liquid handling with image-as-you-add capability
項目符號 True confocal real-time imaging
項目符號 Full-spectrum laser-free illumination
項目符號 96- and 384-multiwell plate and slide imaging
項目符號 Environmentally controlled for live cell experiments
項目符號 Flexible software allowing easy data navigation and classification
項目符號 Integrated binocular viewing
項目符號 High-precision linear x,y, and z motors
項目符號 Motionless stage allowing imaging of suspension or loosely adherent cells without agitating

 

 

 

 

 

 

Applications

 

項目符號 Apoptosis
項目符號 JC1
項目符號 YoPro
項目符號 Annexin V
項目符號 Nuclear fragmentation (DAPI or Hoechst)
項目符號 Cytotoxicity
項目符號 Live/Dead
項目符號 Ethidium homodimer/calcein AM
項目符號 Propidium iodide/fluorescein diacetate
項目符號 Steatosis
項目符號 Mitochondrial Dysfunction

Figure 1.

Measurement of mitochondrial potential changes in M21 cells using TMRE.

Dose response curve of valinomycin activity measured by TMRE and images using the Pathway HT.

TRME

項目符號 NFkB Activation
項目符號 Translocation into nucleus
項目符號 Novel analysis method
項目符號 Ion Channels and Ratioimaging
項目符號 Ca++ (Fluo-4, Fura-2)
項目符號 Na+ (SBFI)
項目符號 Ca++/Na+

Figure 2.

Differential cellular responses in calcium in VR-1 transfected NIH-3T3 cells.

Fura-2 calcium traces of individual cells indicate heterogeneous cellular responses to stimuli.

Cells courtesy Dr. Mike Iadorola, NIH.

Ca++ (Fluo-4, Fura-2)

 

項目符號 cAMP
項目符號 ACT:One™
項目符號 Whole organism/tissue imaging
項目符號 Montage imaging

Figure 3.

High resolution x,y,z positioning system allows precision capture of multiple images allowing imaging of larger specimens at very high resolution.

Montage

 

項目符號 Dual Ratiometric Imaging
項目符號 JC1
項目符號 Calcium (Fura-2)

Figure 4.

Confocal image of mitochondrial potential disruption in HEP-G2 cell line following addition of compound.

Twister 2