Meastro

TissueGnostics Tissue Cytometry for the analysis of cells in tissue and smear

-------An equivalent to Flow Cytometry--------

-----New standard for the entire process of slide scanning and data handling -----

Features overview:

	Pre-scanning to identify tissues, smears and biopsies
	Algorithm for rapid leukocyte detection
	Multi-slide management and movement
	Self adjusting colour and monochrome CCDs
	Automated lens and filter handling
	High speed and high-precision autofocus
	Tissue stitching for overviews of entire sections
	Channel overlay and data storage
	Slide and bar code readout

TissueFAXS-Management and Acquisition Software

	Fully automated slide scanning system
	Preview scan
	Freely selectable ares of interest
	High speed and high-precision autofocus up to 63x oil
	Multi-channel acquisition
	Tissue stitching for overviews of entire sections
	Channel overlay and data storage
	Suitable for cryocut and paraffin sections, cell cutures, TMA’s and smears

TissueStitching-----Image Assembly Software ------

	Large overviews of microscopic specimens.
	Slide overview.
	Superior detail definition.
	Can be used in any combination for digital image-analysis.
	Region-specific evaluation.
	High performance zoom function
	Simple handling of enormous data amounts.

TissueQuest----Cell Analysis Software ----

	Uses novel approach to data acquisition.
	Recognition of individual cells in tissue.
	Measures characteristic staining patterns of cells (e.g. nuclei, ring structures, cytoplasm).
	Generates data in real time.
	Results are analyzed in dot-blot and tabular format.
	Results are further processed based on scattergrams.
	Objective measurements results for superior diagnostic reliability.
	Relative and absolute cell numbers are accurately output.
	Development of innovative therapeutic strategies.
	Fully automated procedures save time and money.
	Compatible with all commercial microscopes and stages.
	User-friendly operation.

HistoQuest----Tissue Analysis Software ----

	Color separation - Extraction of individual channels, showing only one marker at time
	Cell detection
	Reactivity measurement
	Data interpretation
	Results are analyzed in dot-blot and tabular format.

HemoFAXS

HemoFAXS offers fully automated analysis and classification of leukocytes (white blood cells/WBC) based on advanced Support Vector Machine technology. Thanks to a specially designed algorithm, relevant cells are detected in real time. All leukocyte subtypes and normoblasts are accurately differentiated by intelligent segmentation. Classifying leukocytes has never been that easy.

Erythrocyte Morphology

HemoFAXS also offers a well-structured format for rapid analysis of erythrocytes (red blood cells/RBC). Each patient can be screened by single mouse clicks for various RBC diseases.

Numerous Applications

HemoFAXS is an appropriate screening tool for all types of hematological diseases. Both RBC and WBC abnormalities are detected, including leukemia-type alterations.

Cells can be analyzed in blood, bone marrow smears, sputum, cerebrospinal fluid or vaginal smears.

1) Image Recording and Processing

Up to eight patient samples can be simultaneously analyzed. The maximum number of cells per slide can be defined in advance.

2) Classification

The recording command will start the automated process of cell assignment. A specially designed autofocus will ensure that top-level images are recorded. The selected range of images with assigned cells can be manually readjusted at any time.

3) Erythrocyte Morphology

The RBC profile is presented in a well-structured format for speedy analysis.

4) Validation

This screen gives an overview of all patient-related data. The operator can add remarks that will be stored along with the data.

Publications:

Glycogen Synthase Kinase 3β (GSK3β) Regulates Differentiation and Proliferation in Neural Stem Cells from the Rat Subventricular Zone

Martin H. Maurer, Jens O. Brömme, Robert E. Feldmann, Jr., Anne Järve, Fatemeh Sabourl, Heinrich F. Bürgers, Dominik W. Schelshorn, Carola Krüger, Armin Schneider, and Wolfgang Kuschinsky

Journal of Proteome Research 2007, 6, 1198-1208

Is Benign Prostatic Hyperplasia (BPH) an Immunoinflammatory Disease?

Gero Kramer, Dieter Mitteregger, Michael Marberger.

2007, European Urology 51 (2007) 1202-1216

An Improved Method for Discrimination of Cell Populations in Tissue Sections Using Microscopy-Based Multicolor Tissue Cytometry

Rupert C. Ecker, Georg E. Steiner, Katja Österreicher, Radu Rogojanu.

2006, International Society for Analytical Cytology. Cytometry Part A 69A:119-123 (2006)

3D Parallel Coordinate Systems – A New Data Visualization Method in the Context of Microscopy-Based Multicolor Tissue Cytometry (MMTC).

Marc Streit, Rupert C. Ecker, Katja Österreicher, Georg E. Steiner, Horst Bischof, Christine Bangert, Tamara Kopp and Radu Rogojanu.

Cytometry A. 2006 Jul;69(7):601-11

Pimecrolismus leads to an apoptosis-induced depletion of T cells but not Langerhans cells in patients with atopic dermatitis.

Wolfram Hoetzenecker, MD, PhD, Rupert Ecker, PhD, Tamara Kopp, MD, Anton Stuetz, PhD, Georg Stingl, MD, and Adelheid Elbe-Bürger, PhD

2005, Journal of Allergy and Clinical Immunology, 115(6):1276-83
Abstract auf PubMed

Autocrine IL-10 partially prevents differentiation of neonatal dendritic epidermal leukocytes into Langerhans cells.

Souyet Chang-Rodriguez, Rupert C. Ecker, Georg Stingl, Adelhaid Elbe-Bürge

2004, Journal of Leukocyte Biology, 76(3):657-66
Abstract auf PubMed

Spectral Imaging Microscopy: Application in Cytomics and Fluorescence Resonance Energy Transfer (FRET) Analysis.

Rupert C. Ecker, Rainer de Martin, Georg E. Steiner, and Johannes A. Schmid

2004, Cytometry, 59A(2):172-81
Abstract auf PubMed

Multicolor Microscopy-Based Tissue Cytometry at the Single Cell Level.

Rupert C. Ecker and Georg E. Steiner

2004, Cytometry, 59A(2):182-90
Abstract auf PubMed

Corticosteroids but not Pimecrolimus affect viability, maturation and immune function of murine epidermal Langerhans cells.

Wolfram Hoetzenecker, Josef G. Meingassner, Rupert C. Ecker, Georg Stingl, Anton Stuetz, Adelheid Elbe-Buerger

2004, Journal of Investigative Dermatology, 122(3):673-84
Abstract auf PubMed

Tissue Factor Pathway Inhibitor mediates Inhibition of Restenosis by Suppression of Monocyte Chemoattraction and Gelatinase Activity.

Christoph W. Kopp, Thomas Hölzenbein, Sabine Steiner, Rodrig Marculescu, Helga Bergmeister, Daniela Seidinger, Isabella Mosberger, Christoph Kaun, Manfred Cejna, Reinhard Horvat, Johann Wojta, Gerald Maurer, Rupert C. Ecker, Rainer de Martin, Erich Minar

2004, Blood, 103(5):1653-61
Abstract auf PubMed

Overexpression of anti-CD75 reactive proteins on distal and collecting renal tubular epithelial cells in calcium-oxalate stone forming kidneys in Egypt.

Gero Kramer, Georg E. Steiner, Franz Reithmayer, Moshgan Prinz-Kashani, Markus Hohenfellner, Mohamed Gomha, Mohamed Ghoneim, Martin Newman and Michael Marberger

2004, British Journal of Urology International, 93(6):822-826
Abstract auf PubMed

Histopathologische Quantifizierung immunologischer Prozesse.

Rupert C. Ecker, Rainer de Martin, and Georg E. Steiner

2003, Biomedizinische Technik, 48 (Suppl. 1):546-547
Abstract

Cell-suface matrix proteins and sialic acids in cell-crystal adhesion; the effect of cristal binding on the viability of human CAKI-1 renal epithelial cells.

Gero Kramer, Georg E. Steiner, Moshgan Prinz-Kashani, Bernt Bursa, Michael Marberger

2003, British Journal of Urology International, 91:554-559
Abstract auf PubMed

Automated data acquisition by confocal laser scanning microscopy and image analysis of triple stained immunofluorescent leukocytes in tissue.

Georg Erich Steiner, Rupert Christian Ecker, Gero Kramer, Felix Stockenhuber, Michael Johann Marberger

2000, Journal of Immunological Methods, 237(1-2):39-50
Abstract auf PubMed